1.Introduction
Chinese Hamster Ovary Cells (CHO) are currently the most representative engineered cells in the field of recombinant protein production, which have the characteristics of efficient expansion and expression, and the yield of monoclonal antibody in cumber culture can exceed 10g/L. According to statistics, more than 60% of antibody drugs have been developed using CHO engineered cell lines as expression vectors. CHO cell lines include CHO-S, CHO-ZN, CHO-DG44 and CHO-K1, and are widely used for the expression of recombinant DNA proteins.
2.Tofflon CHO complete chemical composition defined serum-free medium
Product Introduction
Tofflon CHO medium series contains three basic medium, CHO-1 CDM08, CHO CDM09 and CHO CDM27, and two feeding products. All of them are completely chemically defined medium without animal source and protein. CHO medium series products are suitable for large-scale serum-free suspension culture of CHO cells and subsequent transfection and expression. It is used in the development and production process of therapeutic protein-related products, which can support high-density cell expansion and stable product expression. Among them, CHO CDM09 has wide adaptability to CHO cell types, CHO-1 CDM08 is more advantageous to CHO-K1, and CHO CDM27 is specially optimized and developed for CHO-ZN cells. In order to adapt to different culture systems and protein production processes, supporting medium products provide corresponding feeding materials, and can also match other commercial feeding products.
Product Information
NO. | Product Name | Type | Catalog No. | Form | Description |
1 | CHO Chemical defined medium(CDM08) | CHO-1 CDM08 | DP08,10L/50L/100L | Dry Powder | Chemical defined, animal origin-free, protein-free medium optimized for the growth of high-density and large-scale suspension cultures of CHO (especially for CHO-K1) cells, and can improve the expression and quality of antibodies and proteins. |
2 | CHO Chemical defined medium(CDM09) | CHO CDM09 | DP09,10L/50L/100L | Dry Powder | Chemical defined, animal origin-free, protein-free medium optimized for the growth of high-density and large-scale suspension cultures of CHO cells (especially for CHO-S) , and can improve the expression and quality of antibodies and proteins. |
3 | CHO Chemical defined medium(CDM27) | CHO CDM27 | DP27,10L/50L/100L | Dry Powder | Chemical defined, animal origin-free, protein-free medium optimized for the growth of high-density and large-scale suspension cultures of CHO cells(especially for CHO-ZN and CHO-S), and can improve the expression and quality of antibodies and proteins. |
4 | Chemical defined feed medium(CDM Feed A/B) | Tobitec FA&Tobitec FB | DPF01-A&DPF01-B,5L/10L/50L | Dry Powder | Chemical defined, animal origin-free, protein-free medium. A highly effective feeding formulation specially designed to improve the growth and production performance of CHO cells can meet the nutritional needs of cell high-density culture and high expression of products. Combined with the characteristics of cell lines, a reasonable feeding strategy can significantly improve cell growth and antibody expression and quality. |
Product Advantages
3.Application of CHO series medium
1.CHO-1 CDM08
2.CHO CDM09
3.CHO CDM27
The Effect of Expression
Tofflon CHO series chemical composition limited medium is a complete chemical composition limited medium without animal source and protein, which can be used for large-scale serum-free suspension culture of cells and subsequent transfection and expression. It is suitable for CHO-S, CHO-Zn, CHO-DG44 and CHO-K1, and is widely used for the expression of recombinant DNA proteins. It has been widely used in biological drug R&D and commercial production.
Tofflon (India) is committed to providing overall pharmaceutical solutions, providing systematic solutions that include key reagents in medium, systematic engineering, core equipment and disposable consumption. In the direction of biological pharmaceutical disposable production, we have four major consumables: key medium reagents, disposable culture bags, fillers and filters. We are committed to providing complete supply support for disposable products. We also set up a localized technical support team in India, build localized technology research and development and experience centers and production plants, and support the development of Indian biological pharmaceutical industry and life science technology with professional technology.
Tofflon provides a full line of key reagent products for life science research and biotechnology development and production. In the field of macromolecular drugs (antibody/protein/vaccine), we provide medium series related products. In the field of cell and gene therapy, we provide a total solution of key reagents for cell and gene therapy technology, such as serum-free medium for culture, cytokines for induction and stimulation, and preservation solutions for freezing storage.